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Sequence-specific recognition of peptide substrates by the low Mr phosphotyrosine protein phosphatase isoforms

✍ Scribed by Monica Bucciantini; Massimo Stefani; Niccolò Taddei; Fabrizio Chiti; Stefania Rigacci; Giampietro Ramponi


Book ID
117108708
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
137 KB
Volume
422
Category
Article
ISSN
0014-5793

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✍ Rainer Gast; Jörn Glökler; Maria Höxter; Michael Kieß; Ronald Frank; Werner Tegg 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 258 KB

A method is described for the elucidation of the peptide substrate phosphorylation specificity of a protein kinase. Peptide libraries with two to six degenerate positions and a length of seven or nine amino acids were generated directly on Sepharose beads by solidphase peptide synthesis according to