✦ LIBER ✦

Sequence similarity of human caliciviruses and small round structured viruses

✍ Scribed by W. D. Cubitt; X. J. Jiang; J. Wang; M. K. Estes

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 685 KB
Volume: 43
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890430311

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The application of reverse transcription‐polymerase chain reaction (RT‐PCR) using primers directed to the RNA dependent RNA polymerase region within ORF1 of Norwalk virus (NV) showed that 31 percent of morphologically typical human caliciviruses (HuCV) and 57% of small round structured viruses (SRSVs) produced a product of 470 bp similar to the NV control, NV 8Flla/68/US. Alignment of the amino acid sequences of morphologically typical HuCVs with previously published sequences for SRSVs, NV, and Snow Mountain agent (SMA) showed a high degree of homology (90–92%) with SMA and a lesser extent of homology with NV (60–61%). The amino acid sequence of two strains of HuCV, HuCV/3C/92/UK, and HuCV/5C/92/UK differed by only one or two amino acids respectively in the RNA dependent RNA polymerase region from that of two strains of SRSV obtained from children in the United Kingdom, SRSV/4S/90/UK and Japan, SRSV/OTH‐25/89/J which were found to have identical amino acid sequences. The use of an EIA for detection of NV antigen employing antisera raised to recombinant NV protein indicated that HuCVs and SRSVs obtained from children and adults in the United Kingdom were antigenically distinct from the prototype Norwalk Virus, NV/8flla/68/US. © 1994 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Small round-structured (Norwalk-like) vi

Small round-structured (Norwalk-like) viruses and classical human caliciviruses in Southeastern Australia

✍ Wright, Peter J.; Gunesekere, Ishara C.; Doultree, Jennifer C.; Marshall, John A 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 160 KB 👁 2 views

A total of 6,226 fecal samples collected from 1980 to 1996 in the Australian states of Victoria, New South Wales, and Tasmania from individuals with gastroenteritis were tested for small round-structured viruses (SRSVs) and classical human caliciviruses (ClHuCVs) by electron microscopy. There were 2

Capsid sequence diversity in small round

Capsid sequence diversity in small round structured viruses from recent UK outbreaks of gastroenteritis

✍ Green, Steve M.; Lambden, Paul R.; Caul, E. Owen; Clarke, Ian N. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB

General Hospigroup I (B447/92/UK) and group II (Yat/94/UK) tal, Southampton.

Molecular characterization of Camberwell

Molecular characterization of Camberwell virus and sequence variation in ORF3 of small round-structured (Norwalk-like) viruses

✍ Cauchi, Mark R.; Doultree, Jennifer C.; Marshall, John A.; Wright, Peter J. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 719 KB

Five small round-structured viruses (SRSVs) associated with gastroenteritis in Victoria, Australia, from January to November 1994 were examined by sequencing cDNA prepared from faecal samples using RT-PCR. The sequence of the 3' half (3.8 kb) of the genome of one of these viruses, Camberwell, was de

Genomic diversity of small round structu

Genomic diversity of small round structured viruses in the United Kingdom

✍ J. P. Norcott; J. Green; D. Lewis; M. K. Estes; K. L. Barlow; D. W. G. Brown 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 640 KB

## Abstract Fifty‐two fecal specimens collected in the United Kingdom between 1986 and 1992, which contained small round structured virus (SRSV) particles, were tested by reverse transcriptase polymerase chain reaction assays using two primer pairs derived from sequences of Snow Mountain Agent and

Epidemiologic applications of novel mole

Epidemiologic applications of novel molecular methods to detect and differentiate small round structured viruses (Norwalk-like viruses)

✍ Tamie Ando; Qi Jin; Jon R. Gentsch; Stephan S. Monroe; Jacqueline S. Noel; Scott 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 794 KB

## Abstract The molecular epidemiology of a large, multi‐state outbreak of oyster‐associated gastroenteritis [Kohn et al. (1995): __Journal of the American Medical Association 273__:466–471. Dowell et al. (1995): __Journal of Infectious Diseases 171__:1497–1503.] was examined using new methods to d

Molecular characterisation of small roun

Molecular characterisation of small round structured viruses associated with gastroenteritis in South Africa

✍ Mrs. Marianne Wolfaardt; Maureen B. Taylor; Wilhelm O. K. Grabow; W. David Cubit 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 575 KB

## Abstract The application of the reverse transcriptase poly‐ merase chain reaction (RT‐PCR) has enabled several morphologically and physically similar small round structured viruses (SRSVs), including the prototype Norwalk virus (NV), to be classified within the __Caliciviridae__. This technique,