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Sequence-selective extraction of single-stranded DNA using DNA-functionalized reverse micelles

โœ Scribed by Maruyama, Tatsuo; Hosogi, Takuya; Goto, Masahiro


Book ID
121276389
Publisher
Royal Society of Chemistry
Year
2007
Tongue
English
Weight
690 KB
Volume
43
Category
Article
ISSN
1359-7345

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A simple procedure for the precise assembly of functional DNA sequences from overlapping fragments is described. The fragments to be joined are cloned in tandem in the proper relative orientation into a vector from which single-stranded DNA copies can be obtained. Single-stranded DNA is cut by a res