Sequence of the bifunctionalade1gene in the purine biosynthetic pathway of the fission yeastSchizosaccharomyces pombe

✍ Scribed by Rod McKenzie; Peter Schuchert; Brian Kilbey

• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 681 KB
• Volume: 12
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00368061

✦ Synopsis

The adel gene of the fission yeast Schizosaccharomyces pombe encodes a bifunctional polypeptide with glycinamide ribotide synthetase (GARSase) and aminoimidazole ribotide synthetase (AIRSase) enzeyme activities. These enzyme activities carry out the 2nd and 5th steps, respectively, of the purine synthetic pathway. We report the cloning of the adel gene on a 4.4 kb Sau3A insert in the yeast shuttle vector pWH5. Integration of this genomic insert at or near the adel locus and its ability to complement, by transformation, three different types of adel mutants proved that it contains the adel chromosomal gene. Analysis of the nucleotide sequence of this insert revealed the presence of an uninterrupted open reading frame of 2,367 pb. This sequence, and the predicted 789 amino acid sequence encoded, both show a high degree of homology with the functionally equivalent ade5,7 gene sequence of Saccharomyces cerevisiae (approx. 60% overall in both cases) and Gart gene sequences of Drosophila melanogaster. The size of the adel RNA transcript is about 2.7 kb.