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Sequence of choline acetyltransferase temperature-sensitive mutants determined by the polymerase chain reaction

✍ Scribed by Tajima, Y.; Salvaterra, P.M.

Book ID
123167576
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
818 KB
Volume
39
Category
Article
ISSN
0306-4522

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Direct sequencing of tobacco chloroplast
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We have developed a polymerase chain reaction (PCR) method for sequencing of tobacco chloroplast genome. In a mixture containing chloroplast DNA, 5'-end-labeled oligonucleotide primer, Taq DNA polymerase and reaction buffer, we were able to sequence a segment of chloroplast 16S rRNA gene. The result