Sequence-induced DNA curvature at the bacteriophage λ origin of replication
✍ Scribed by Zahn, Kenneth; Blattner, Frederick R.
- Book ID
- 109741395
- Publisher
- Nature Publishing Group
- Year
- 1985
- Tongue
- English
- Weight
- 356 KB
- Volume
- 317
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/317451a0
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The purified bacteriophage 2 replication proteins O and P sediment separately in metrizamide gradients of low ionic strength as dimers. Together they interact with each other forming an oligomer, composed of two molecules of 20 and one molecule of ,~P. The 20-P oligomer is active in the in vitro rep
By means of a nitrocellulose filter binding assay, DNA binding activities among proteins fractionated from extracts of Escherichia coli carrying 2dv have been surveyed. An activity was found that binds specifically to a fragment of 164 base pairs that specifies the 2 replication origin (2 ori). This