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Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322

โœ Scribed by Nguyen, Toai T.; Postle, Kathleen; Bertrand, Kevin P.


Book ID
122860060
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
1003 KB
Volume
25
Category
Article
ISSN
0378-1119

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In vitro recombination techniques were used to construct hybrid plasmids between pBR 322 and different DNA fragments derived from pML 21 and the E. coli chromosome. Some of the resulting hybrid plasmids express the tetracycline resistance gene of pBR 322, depending on the DNA fragment which has been