Sequence determination of a transcribed rabbit class II gene with homology toHLA-DQα

✍ Scribed by Christian LeGuern; Jocelyn D. Weissman; Patrice N. Marcher; Evelyne Jouvin-Marcher; Anne Laverrière; Margherita R. Bagnato; Thomas J. Kindt

• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 495 KB
• Volume: 25
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00364275

✦ Synopsis

The human HLA-DQ~ probe was used to screen genomic and cDNA libraries constructed from a rabbit Tcell line. Clones containing highly homologous sequences were obtained from both libraries and their sequences were determined. The organization of the RLA-DQ gene was determined by comparison of the nucleotide sequences of the genomic clone to that of the corresponding cDNA clone. This analysis allowed assignment of the complete structure of the RLA-DQ~ chain. Comparisons with human and mouse class II products revealed that RLA-DQ is more closely related to HLA-DQ/DXthan to H-2 A. In contrast to the DQ/DX region of man, which contains at least two distinct alpha genes, the rabbit genome contains a single DQ gene which is equally distant from the HLA-DQ or -DX genes. The rabbit DQ~ gene, like human I, tLA-DQ, is transcribed in T cells.