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Sequence and structural analysis of the 5′ noncoding region of hepatitis C virus in patients with chronic infection

✍ Scribed by Flávio Marcos Gomes Araújo; Ariane Machado-Lima; Alan Mitchell Durham; Rosangela Teixeira; Guilherme Oliveira

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
212 KB
Volume
81
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Hepatitis C virus (HCV), exhibits considerable genetic diversity, but presents a relatively well conserved 5′ noncoding region (5′ NCR) among all genotypes. In this study, the structural features and translational efficiency of the HCV 5′ NCR sequences were analyzed using the programs RNA__fold__, RNA__shapes__ and RNA__pdist__ and with a bicistronic dual luciferase expression system, respectively. RNA structure prediction software indicated that base substitutions will alter potentially the 5′ NCR structure. The heterogeneous sequence observed on 5′ NCR led to important changes in their translation efficiency in different cell culture lines. Interactions of the viral RNA with cellular transacting factors may vary according to the cell type and viral genome polymorphisms that may result in the translational efficiency observed. J. Med. Virol. 81:1212–1219, 2009. © 2009 Wiley‐Liss, Inc.

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