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Sequence and organization analyses of aZygosaccharomyces rouxii DNA fragment containing theHIS3 gene

✍ Scribed by Sychrova, Hana; Braun, Valerie; Souciet, Jean-Luc

Book ID
102648045
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
700 KB
Volume
16
Category
Article
ISSN
0749-503X

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✦ Synopsis

The nucleotide sequence of a 3.4 kb fragment containing the HIS3 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The fragment was cloned from a Z. rouxii genomic DNA library by complementation of a Saccharomyces cerevisiae his3 mutant strain. The sequenced DNA fragment contained three open reading frames; the middle one (678 bp long, predicting a protein of 226 amino acids) shared a high degree of similarity with HIS3 genes of other yeast species. In the promoter region of the putative ZrHIS3 gene, a T c element required for constitutive transcription was found. The GenBank Accession No. of the sequenced DNA region is Y18561.

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We have isolated the Candida albicans HIS4 (CaHIS4) gene by complementation of a his4-34 Saccharomyces cerevisiae mutant. The sequenced DNA fragment contains a putative ORF of 2514 bp, whose translation product shares a global identity of 44% and 55% to the His4 protein homologs of S. cerevisiae and