✦ LIBER ✦

Sequence and expression of the gene for N10-formyltetrahydrofolate synthetase from Clostridium cylindrosporum

✍ Scribed by Carolyn A. Rankin; Gale C. Haslam; Richard H. Himes

Publisher: Cold Spring Harbor Laboratory Press
Year: 2008
Tongue: English
Weight: 798 KB
Volume: 2
Category: Article
ISSN: 0961-8368
DOI: 10.1002/pro.5560020208

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✦ Synopsis

Abstract

Sau3 A and Hind III restriction fragments of Clostridium cylindrosporum genomic DNA were used to isolate clones containing 80% of the N^10^‐H~4~folate synthetase gene in a 5′ fragment and the remaining 20% of the gene in the 3′ fragment. These fragments were joined at a common SnaB I restriction site and expressed in Escherichia coli at a level equivalent to what is normally found in C. cylindrosporum. Sequence comparisons show a large degree of homology with genes from two other clostridial species, including a thermophile. Certain conserved sequences found in the three clostridial proteins and in the N^10^‐H~4~folate synthetase portion of eukaryotic Cl‐H~4~folate synthases may represent consensus sequences for nucleotide and H~4~folate binding.

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