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Sequence analysis of the larval βII-globin gene ofXenopus laevis

✍ Scribed by Wolfgang Meyerhof; Manfred Köster; Jürg Stalder; Rudolf Weber; Walter Knöchel

Publisher
Springer
Year
1986
Tongue
English
Weight
548 KB
Volume
11
Category
Article
ISSN
0301-4851

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✦ Synopsis

The 1822 bp sequence of the larval Xenopus laevis/31rglobin gene is reported together with 240 bp upstream of the gene and 190 bp beyond the site of polyadenylation. The mRNA start point was determined by primer extension as well as nuclease $1 mapping and the polyadenylation site by comparison of the gene sequence to the mRNA sequence derived from a corresponding cDNA clone. Like other vertebrate globin genes, this gene comprises three exons interrupted by two intervening sequences (IVS). IVS I spans over 582 nucleotides and interrupts the exon sequences within codon 30. IVS II is located between the codons 104/105 and spans over 617 nucleotides.

The 5' region of the gene contains the canonical TATAA homology at position -31. Comparison of the upstream sequence to that of Xenopus laevis larval/3i-globin gene revealed a conserved sequence, located between nucleotide positions -60 and -87, which might function as regulatory element of transcription. Whereas the upstream region of the larval/31rglobin gene does not contain a CAAT box, we notice a reiterated AAATGA motif and discuss its possible significance.

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