We describe here the construction of six deletion mutants and their basic phenotypic analysis in three different backgrounds. The six genes were disrupted in three diploid strains (FY1679, W303 and CEN.PK2) by the long flanking homology (LFH) method (Wach, 1996). Transformants were selected as genet
Sequence Analysis of the 33 kb Long Region BetweenORC5 andSUI1 from the Left Arm of Chromosome XIV fromSaccharomyces cerevisiae
✍ Scribed by Sen-Gupta, Mark; Güldener, Ulrich; Beinhauer, Jens; Fiedler, Thomas; Hegemann, Johannes H.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 238 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
We have determined the nucleotide sequence of a chromosomal region of 33,016 bp located on the left arm of chromosome XIV from budding yeast between the ORC5 and the SUI1 gene. Subsequent sequence analysis revealed the presence of 18 non-overlapping open reading frames (ORFs) including eight previously identified and sequenced genes (ORC5, ATX1, SIP3, NRD1, RAD50, MPA43, RPA49 and SUI1). Three other ORFs (YNL256w, YNL255c and YNL247w) code for putative proteins with significant homology to proteins from other organisms, while 4 ORFs exhibit only weak homology to known proteins. Three ORFs have no homology with sequences in the databases.
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