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Sequence analysis of linked maize 22 kDa α-zein genes

✍ Scribed by Gary A. Thompson; David R. Siemieniak; Leang C. Sieu; Jerry L. Slightom; Brian A. Larkins

Publisher: Springer
Year: 1992
Tongue: English
Weight: 535 KB
Volume: 18
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00020030

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✦ Synopsis

We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa ~-zein genes. The 5' gene (gZ22.8) encodes a complete polypeptide and contains putative regulatory sequences in both the 5' and 3' flanking regions that are typical of zein genes. In contrast, the 3' gene (~gZ22.8) appears to be a pseudogene, because it contains numerous insertions and deletions that would prevent translation of the mRNA. Alignment of the 5' and 3' flanking sequences of both genes indicated that they resulted from a 3.3 kb DNA duplication event.

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