Sequence analysis and HLA-DR genotyping of a novelHLA-DRw14allele

✍ Scribed by Fumiya Obata; Ichiro Ito; Koichi Ito; Akio Abe; Midori Ohkubo; Keiji Onda; Yong-guang Yang; Koji Watanabe; Noboru Kashiwagi

• Publisher: Springer-Verlag
• Year: 1990
• Tongue: English
• Weight: 686 KB
• Volume: 32
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00211645

✦ Synopsis

Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401 (DRw14-Dw9) and DRB1*1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) was amplified enzymatically and sequenced after cloning into a plasmid vector. The amino acid sequence of the first domain in the DR beta 1 chain encoded in the DRB1-14c allele was more similar to that of the DRB1*1401 allele (three amino acid substitutions) than to that of the DRB1*1402 allele (six amino acid substitutions). No polymorphic amino acid residue that could explain the common serologic HLA-DRw14 specificity was identified among the sequences of the three DRw14-related alleles. Sequence-specific oligonucleotides (SSOs) were synthesized on the basis of the DRB1-14c nucleotide sequence and used for genotyping of the Japanese population. These SSOs served as useful probes for identifying the DRB1-14c allele in a wide range of donors.