Separation system for fractionation of polyacrylamide by gel permeation chromatography

✍ Scribed by R. Biran; J.V. Dawkins

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 450 KB
• Volume: 20
• Category: Article
• ISSN: 0014-3057
• DOI: 10.1016/0014-3057(84)90198-8

✦ Synopsis

Samples of polyacrylamide were prepared by radical polymerisation of monomer in aqueous solution. Fractions of polyacrylamide were produced by a fractional precipitation technique with methanol as non-solvent. Molecular weights of the polyacrylamides were determined by solution viscometry. Analytical gel permeation chromatography (GPC) of the polyacrylamide samples and fractions was performed with porous silicas having a chemically bonded aminopropyl phase and with an eluenI consisting of formamide-water (1:5). The GPC retention results indicated the successful separation of polyacrylamides having molecular weights in the range 104-106. GPC of polyacrylamide with this separation system appeared to be dominated by a steric exclusion mechanism.