Separation Processes in Biotechnology

✍ Scribed by Asenjo, Juan A

• Publisher: CRC Press
• Year: 2020
• Tongue: English
• Leaves: 824
• Edition: First edition
• Category: Library

✦ Synopsis

Series Introduction -- Foreword (Alan S. Michaels) -- Preface -- I Separation Processes -- 1 Selection of Operations in Separation Processes /Juan A. Asenio -- 2 Chemical, Physical, and Biochemical Concepts in Isolation and Purification of Proteins /Milton T. W. Hearn and Birger Anspach -- II Product Release and Recovery -- 3 Protein Secretion Systems in Microbial and Mammalian Cells /Donald T. Moir and Jen-I Mao -- 4 Cell Disruption by Homogenizer /Cady R. Engler -- 5 Bead Mill Disruption /Horst Schutte and Maria-Regina Kula -- 6 Enzymatic Cell Lysis for Product Release /Juan A. Asen;o and Barbara A. Andrews -- 7 Chemical Permeabilization of Cells for Intracellular Product Release /Thomas J. Naglak, David J. Hettwer, and Henry Y. Wang -- III Concentration and Separation Operations -- 8 Membrane Systems /A. G. Fane and J. M. Radovich -- 9 Ion -Exchange Processes /Charles D. Shuey -- 10 Aqueous Two-Phase Separations /Per-Jke Albertsson, Cote Johansson, and Folke T jerneld -- 11 Precipitation /Charles E. Glatz -- IV Purification Operations -- 12 Ion Exchange in Purification /Nien-Hwa Linda Wang -- 13 Process Affinity Chromatography /Yannis D. Clonis -- 14 Reversed-Phase and Hydrophobic Interaction Chromatography of Peptides and Proteins /Ziad El Rossi, Ann L. Lee, and Csaba Horvath -- 15 Analysis of Effluent Profiles in Large-Scale Liquid Chromatography /J. Hong -- 16 Electrically Driven Separation Processes: Analytical and Preparative Methods /Cornelius F. Ivory -- V Design of Separation Processes -- 17 Synthesis of Downstream Processes /George J. Prokopakis and Juan A. Asenjo -- 18 Automation in Protein Purification: The Use of Expert Systems for Control of Process Chromatography /Andrew C. Kenney -- 19 Downstream Processing Plant and Equipment /M. P. Brocklebank -- 20 Downstream Process Economics /Rajiv Datar and Carl-Gustaf Rosen -- Index.;Edited to avoid duplication and favor comprehensiveness, 20 contributors detail the recovery, separation, and purification operations of bioprocess technology. Individual chapters in this classic yet still highly relevant work emphasize concepts that are becoming more and more important when applied to the large scale versions of techniques that are considered well established. Aside from fully discussing processes, Separation Processes in Biotechnology includes sections on concentration separation and operation, purification operations, and product release and recovery. It also discusses plant operation and equipment and delves into economic considerations

✦ Table of Contents

Cover......Page 1
Half Title......Page 2
Series Page......Page 3
Title Page......Page 6
Copyright Page......Page 7
Series Introduction......Page 8
Foreword......Page 10
Preface......Page 12
Table of Contents......Page 16
I: Separation Processes......Page 24
1 Selection of Operations in Separation Processes......Page 26
2 Chemical, Physical, and Biochemical Concepts in Isolation and Purification of Proteins......Page 40
II: Product Release and Recovery......Page 88
3 Protein Secretion Systems in Microbial and Mammalian Cells......Page 90
4 Cell Disruption by Homogenizer......Page 118
5 Bead Mill Disruption......Page 130
6 Enzymatic Cell Lysis for Product Release......Page 166
7 Chemical Permeabilization of Cells for Intracellular Product Release......Page 200
III: Concentration and Separation Operations......Page 230
8 Membrane Systems......Page 232
9 Ion-Exchange Processes......Page 286
10 Aqueous Two-Phase Separations......Page 310
11 Precipitation......Page 352
IV: Purification Operations......Page 380
12 Ion Exchange in Purification......Page 382
13 Process Affinity Chromatography......Page 424
14 Reversed-Phase and Hydrophobic Interaction Chromatography of Peptides and Proteins......Page 470
15 Analysis of Effluent Profiles in Large-Scale Liquid Chromatography......Page 518
16 Electrically Driven Separation Processes: Analytical and Preparative Methods......Page 540
V: Design of Separation Processes......Page 592
17 Synthesis of Downstream Processes......Page 594
18 Automation in Protein Purification: The Use of Expert Systems for Control of Process Chromatography......Page 626
19 Downstream Processing Plant and Equipment......Page 640
20 Downstream Process Economics......Page 764
Index......Page 818

✦ Subjects

Biomolecules--Separation;Biotechnology--Technique;SCIENCE / Biotechnology;SCIENCE / Chemistry / General;SCIENCE / Chemistry / Industrial & Technical;Electronic books;Biomolecules -- Separation;Biotechnology -- Technique