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Separation, pre-concentration, and HPLC analysis of methylxanthines in urine samples

✍ Scribed by Mirosława Zydroń; Jacek Baranowski; Irena Baranowska

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
803 KB
Volume
27
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

An SPE method, using RP18 phases, for the simultaneous extraction of caffeine, theobromine, theophylline, paraxanthine, 1‐methylxanthine, 3‐methylxanthine, 7‐methylxanthine, 1‐methyluric acid, 1,3‐dimethyluric acid, 1,7‐dimethyluric acid, and 1,3,7‐trimethyluric acid from urine has been developed. Besides a gradient HPLC system for the analysis of the compounds of interest on a LiChrosorb RP‐18 (7 μm) column with mobile phase containing 0.05% aq. solution of trifluoroacetic acid and acetonitrile has been elaborated. The procedure has been successfully applied to the analysis of methylxanthines and methyluric acids in urine of patients with chronic asthma treated with theophylline and in urine of healthy subjects.

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