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Separation of vitamin B complex and folic acid using TLC plates impregnated with some transition metal ions

✍ Scribed by Ravi Bhushan; Vineeta Parshad

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 238 KB
Volume: 8
Category: Article
ISSN: 0269-3879
DOI: 10.1002/bmc.1130080411

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✦ Synopsis

The separation of Vitamin B complex and folk acid was achieved on silica gel layers impregnated with Mn++, Fe++, Co++, Ni++, Cu++, Cd++, Zn++ or Mg++ (0.1Y0, 0.2Y0, 0.3%, 0.4% of each ion). Three new solvent systems n-propanol: n-butanol : water : ammonia (7 : 5 : 1 : 2, by vol), n-propanol : n-butanol: water : ammonia (7 : 5 : 1 : 1.5, by vol), n-propanol : n-butanol : water : ammonia (7: 5 : 0.75: 2, by vol) were developed. The spots of vitamins were located by exposing the chromatoplate to iodine vapours.

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