Separation of the optical isomers of a new 1,4-dihydropyridine calcium channel blocker (LF 2.0254) by liquid and supercritical fluid chromatography

✍ Scribed by Laurent Siret; Dr. Pierre Macaudiere; Nathalie Bargmann-Leyder; André Tambute; Marcel Caude; Émile Gougeon

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 479 KB
• Volume: 6
• Category: Article
• ISSN: 0899-0042
• DOI: 10.1002/chir.530060513

✦ Synopsis

The four optical isomers of a new calcium channel blocker LF 2.0254 (developed by Laboratoires Fournier) are resolved by chromatography using chiral stationary phases. Two methods are proposed: the first one involving two chiral stationary phases

Methods

Apparatus LC was performed with a modular liquid chromatograph (Gilson, Villiers-le-Bel, France) comprising a Model 303 pump, a Model 802 manometric module, a Model 811 dynamic mixer (1.5 ml) and a UV-116 variable-wavelength detector (190-350 nm). All results were recorded with a Shimadzu C-R3A integrator (Touzart et Matignon,