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Separation of the integral membrane glycoproteins E1 and E2 of semliki forest virus by affinity chromatography on concanavalin A-Sepharose

โœ Scribed by Kari Mattila


Book ID
113126023
Publisher
Elsevier Science
Year
1979
Weight
604 KB
Volume
579
Category
Article
ISSN
0005-2795

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Rapid quantitative separation of the maj
โœ Y. Shami; S. Ship; A. Rothstein ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 449 KB

Several methods were tested for separating the major glycoproteins from other proteins extracted from human red cell membranes by the nondenaturing detergent, Triton X-100. Separation could be achieved by isoelectric focusing, but the intrinsic proteins (predominantly band 3) become irreversibly pre