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Separation of supercoiled and open-circular plasmid DNA by liquid-liquid counter-current chromatography

โœ Scribed by D. Kendall; A.J. Booth; M.S. Levy; G.J. Lye


Book ID
110290594
Publisher
Springer Netherlands
Year
2001
Tongue
English
Weight
78 KB
Volume
23
Category
Article
ISSN
0141-5492

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## Abstract In the current study we developed a process for the capture of pDNA exploiting the ability of aqueous twoโ€phase systems to differentiate between different forms of DNA. In these systems scpDNA exhibits a near quantitative partitioning in the saltโ€rich bottom phase. The successive recove