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Separation of Spliceosome Assembly from Catalysis with Caged pre-mRNA Substrates

✍ Scribed by Steven G. Chaulk; Andrew M. MacMillan


Book ID
101368004
Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
112 KB
Volume
113
Category
Article
ISSN
0044-8249

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Separation of Spliceosome Assembly from
✍ Steven G. Chaulk; Andrew M. MacMillan πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 112 KB

Getting spliced? Pre-mRNA splicing is catalyzed by the spliceosome, a complex assembly of proteins and RNA, which forms in an ordered fashion on the substrate. If one of the residues of the substrate is caged with a photolabile o-nitrobenzyl group, the splicing reaction can be transiently blocked, u