Separation of some DL-amino acids into their optical isomers by paper adsorption chromatography

✍ Scribed by C. L. de Ligny; H. Nieboer; J. J. M. de Vijlder; J. H. H. G. van Willigen

• Book ID: 104586588
• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 374 KB
• Volume: 82
• Category: Article
• ISSN: 0165-0513
• DOI: 10.1002/recl.19630820214

✦ Synopsis

Abstract

A systematic research was made into the conditions producing optimum separation of the optical isomers of amino acids by paper chromatography, using as pilot substances racemic mixtures of the amino acids histidine, tryptophan and kynurenin and methanol‐water mixtures as eluents. The optimum separations, i.e. the smallest ratios of the moving‐rates of the slow and the fast component, were obtained by elution with a 90 vol% methanol‐water mixture at as low a temperature as possible (in our case −15°). In this way the separation of the optical isomers of dl‐cystine by paper chromatography, which has not yet been reported, could be accomplished.