Separation of bases, ribonucleosides and
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Bimal C. Pal; James D. Regan; Franklin D. Hamilton
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Article
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1975
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Elsevier Science
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English
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CDP and CTP are used as substrates in the assay of ribonucleotide reductase. deaminase and nucleosidase activity in crude enzyme preparations. After incubation. the nucleotides are hydrolyzed to nucleosides by sequential treatment with potato apyrase and alkaline phosphatase. An aliquot is then chro