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Separation of ribonucleotides and deoxyribonucleotides on columns of borate covalently linked to cellulose. Application to the assay of ribonucleoside diphosphate reductase

โœ Scribed by Moore, E. Colleen; Peterson, Dolores; Yang, Li Ying; Yeung, Chou Yau; Neff, Norma F.


Book ID
125968805
Publisher
American Chemical Society
Year
1974
Tongue
English
Weight
443 KB
Volume
13
Category
Article
ISSN
0006-2960

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Separation of bases, ribonucleosides and
โœ Bimal C. Pal; James D. Regan; Franklin D. Hamilton ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 477 KB

CDP and CTP are used as substrates in the assay of ribonucleotide reductase. deaminase and nucleosidase activity in crude enzyme preparations. After incubation. the nucleotides are hydrolyzed to nucleosides by sequential treatment with potato apyrase and alkaline phosphatase. An aliquot is then chro