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Separation of ribonucleosides and ribonucleotides by a one-dimensional paper chromatographic system

✍ Scribed by Marion Nestle; Walden K. Roberts

Book ID
107710928
Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
599 KB
Volume
22
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

A modified two-dimensional paper chromat
A modified two-dimensional paper chromatographic system for the separation of DNP-amino acids
✍ Frederick W. Pairent; Martin B. Williamson πŸ“‚ Article πŸ“… 1960 πŸ› Elsevier Science 🌐 English βš– 268 KB

The 0.05 M phthslatc buffer was preparecl by mixing 50 ml of 0.1 M potassium acicl phthalato and 45.45 ml of 0.1 N NsOH and diluting to IOO ml with CO,-free waferG. l \* All the DNP-amino acicls used in this study were preparccl by the method of SANGEIZ~ or that of LEVY AND CHTJNG'.

Separation of bases, ribonucleosides and
Separation of bases, ribonucleosides and deoxyribonucleosides by anion-exclusion and partition chromatography on cation-exchange resin: Application to the assay of ribonucleotide reductase, deaminase and nucleosidase
✍ Bimal C. Pal; James D. Regan; Franklin D. Hamilton πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 477 KB

CDP and CTP are used as substrates in the assay of ribonucleotide reductase. deaminase and nucleosidase activity in crude enzyme preparations. After incubation. the nucleotides are hydrolyzed to nucleosides by sequential treatment with potato apyrase and alkaline phosphatase. An aliquot is then chro