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Separation of racemic 2,4-dinitrophenyl amino acids on zirconia-immobilized quinine carbamate in reversed-phase liquid chromatography

✍ Scribed by Jung Hag Park; Joon Woo Lee; Young Tae Song; Chun Sup Ra; Jin Soon Cha; Jae Jeong Ryoo; Wonjae Lee; In Whan Kim; Myung Duk Jang

Book ID
102924470
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
609 KB
Volume
27
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Zirconia is known to be one of the best chromatographic support materials due to its excellent chemical, thermal, and mechanical stability. A quinine carbamate‐coated zirconia was prepared as a chiral stationary phase for separation of enantiomers of DNP‐amino acids in reversed‐phase liquid chromatography. Retention and enantioselectivity of this phase were compared to those for quinine carbamate bonded onto silica. Most amino acids studied were separated on the quinine carbamate‐zirconia CSP although retention was longer and chiral selectivity was somewhat lower than on the corresponding silica CSP. Increased retention and decreased selectivity are probably due to strong non‐enantioselective Lewis acid‐base interactions between the amino acid molecule and the residual Lewis acid sites on the zirconia surface.

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