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Separation of peripheral blood lymphocytes from an individual mouse for functional studies

✍ Scribed by Phillip Chandler

Book ID: 103582104
Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 60 KB
Volume: 1
Category: Article
ISSN: 0167-5699
DOI: 10.1016/0167-5699(80)90033-x

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Cover Picture: The cover image features

Cover Picture: The cover image features a frozen section from an embryonic day 16 mouse spleen stained by immunofluorescence to demonstrate the presence and location of lymphoid tissue inducer (LTi) cells. Withers et al. (pp. 3240–3245) show that these cells form the focus of lymphocyte accumulation in the developing mouse spleen. Red staining for CD45 marks haemopoietic cells, green shows IL-7Rα expression, and CD4 expression is in blue. LTi cells that express all markers (in white) cluster around blood vessels in the embryonic spleen where signalling to stromal cells initiates lymphocyte recruitment. – Eur. J. Immunol. 11/2007

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