Separation of peptides on cellulose phosphate

✍ Scribed by W.R. Holmquist; W.A. Schroeder

• Book ID: 104147210
• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 501 KB
• Volume: 26
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98904-6

✦ Synopsis

Cellulose phosphate cation exchange powders have received little attention as a means for the column chromatographic isolation and purification of proteins and peptides. MARSHALL et aZ.l used cellulose phosphate for the isolation of carbamyl phosphate synthetase from frog livers with succinate buffer as tlie eluent. MITZ AND IOO cm chromatographic column was poured in a single section with this suspension.

Eq&ibration and loading of colwnn

The column was equilibrated at 28" with 250 ml of buffer 33. The mixture of peptides, which contained up to 50 pmoles of each of several peptides (the maximum * Contribution No. 3390.