✦ LIBER ✦
Separation of nuclear cAMP independent protein kinases NI and NII from their chromosomal protein substrates and enzyme inhibitors by the use of a casein-phosvitin-Sepharose column
✍ Scribed by Marc Delpech; Florence Levy-Favatier; Jacques Kruh
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 573 KB
- Volume
- 152
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A casein-phosvitin-Sepharose chromatography column allows separation of nuclear protein kinases from their chromosomal phosphoprotein substrates and from at least some protein kinase inhibitors in a single step. The additional step of passing the eluted material through a partially hydrolyzed, dephosphorylated casein-Sepharose column separates the two protein kinases, NI and NII, from each other.