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Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P and its metabolites by micellar electrokinetic chromatography

✍ Scribed by Anita L. Freed; Susan M. Lunte

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 131 KB
Volume: 21
Category: Article
ISSN: 0173-0835
DOI: 10.1002/1522-2683(20000601)21:10<1992::aid-elps1992>3.0.co;2-e

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✦ Synopsis

This paper describes the development of analytical methodology for the separation of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized substance P (CBI-SP) and five lysine-containing metabolites by micellar electrokinetic chromatography (MEKC). The effect of surfactant composition and organic modifiers on the separation was investigated. The final separation buffer consisted of 80 mM sodium cholate in 50 mM N-tris (hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES), pH 7. All six lysine-containing peptides were separated under these conditions.