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Separation of myosin light chains by high-speed gel filtration on TSK-GEL SW columns

โœ Scribed by Shugo Watabe; Kanehisa Hashimoto; Tohru Takamatsu


Book ID
103147546
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
482 KB
Volume
260
Category
Article
ISSN
1873-3778

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High-performance gel permeation chromato
โœ G.Brent Irvine; Christopher Shaw ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 631 KB

Trifluoroacetic acid (0.1% w/v) is an excellent solvent for polypeptides, is volatile, and has a low absorbance of ultraviolet light of low wavelength. Polypeptides subjected to chromatography on columns of TSK-G2000-SW or TSK-G3000-SW in this solvent were eluted as sharp peaks. Retention volume was