Separation of metal cations in acidic solution by capillary electrophoresis with direct and indirect UV detection

✍ Scribed by Thornton, Michelle J. ;Fritz, James S.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 398 KB
• Volume: 20
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240201207

✦ Synopsis

Abstract

Methods for the detectionn of metal cations under acidic conditions, near PH 2, in capillary electrophoresis (CE) were investigatged. Conditions for direct UV detection of UV absorbing metal cations such as Cr^3+^, Cu^2+^, Fe^3+^, UO~2~^2+^, VO^2+^, and VO^2+^ were established With aqueous HCl or HClO~4~ as the electroyte carrier. The speciation of vanadium(IV) and vanadium(V) at PH 2.3 by CE was achieved with direct detection at 185 nm. With the strong absorbance at 185 nm, no complexation was needed to detect the metal cations. An indirect UV detrection scheme for acidic conditions was also investigated. Several background carrier electolytes (BCES) were studied including 4‐methylbenzylamince, nicontinamide, pyridazine, guanidine, 3‐picoline, and chromium (III) to determine their effectivencess under very acidic conditions. The efect of ioni c surfactants and the nonionic surfactant, Trition X‐100, on the peak heights and N Values was also studied.