Separation of immunoreactive lymphocytes from human pluripotent stem cells (CFU-GEMM) by means of counterflow centrifugation
✍ Scribed by Witte, T. ;Koekman, E. ;Geestman, E. ;Plas, A. ;Blankenborg, G. ;Wessels, J. ;Haanen, C.
- Publisher
- Springer-Verlag
- Year
- 1984
- Weight
- 449 KB
- Volume
- 48
- Category
- Article
- ISSN
- 1432-0584
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✦ Synopsis
Counterflow centrifugation with continuous monitoring of the output for cell number and cell scatter was used to separate low density (d less than 1.070 g/ml) human bone marrow cells in two fractions: one containing the majority of small size lymphocytes and the other the majority of the larger sized committed progenitor cells. The recovery of the pluripotent stem cells (CFU-GEMM) in the large cell fraction was complete. The mitogenic reactivity of this putative stem cell fraction had decreased to 6% and 11%, of the original value as measured with phytohemagglutinin stimulation and one way mixed lymphocytic culture respectively. Counterflow centrifugation offers a physical separation technique, by which the majority of the immunoreactive cells can be separated from the pluripotent hematopoietic stem cells.