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Separation of Human proα1(I) and proα2(I) Procollagen Chains by Reverse Phase High Performance Liquid Chromatography

✍ Scribed by Michel Van Der Rest; Catherine A. Stolle; Darwin J. Prockop; Peter P. Fietzek

Book ID
114178960
Publisher
Elsevier
Year
1982
Weight
605 KB
Volume
2
Category
Article
ISSN
0174-173X

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The zeta (0 and various gamma (y) chains of Hb Portland I have been separated and isolated from blood samples obtained from neonates with hydrops fetalis due to homozygous a-thalassemia. By using developers containing acetonitrile, methanol, and potassium phosphate and either an analytical (3.9 mm X