Separation of hemoglobin and myoglobin from yellowfin tuna red muscle by ultrafiltration: Effect of pH and ionic strength

✍ Scribed by F. Sannier; C. LeCoeur; Q. Zhao; I. Garreau; J. M. Piot

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 499 KB
• Volume: 52
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19961120)52:4<501::aid-bit5>3.0.co;2-t

✦ Synopsis

Efficiency and selectivity of 30 and 150 kd inorganic ultrafiltration membranes (Techsep) toward tuna hemoglobin and myoglobin were studied. The influence of pH and ionic strength was investigated. Mass flow of myoglobin was higher at its isoelectric pH (8.6) and for low ionic strength (1.5 mM). This result was related to the absence of electrostatic repulsion between myoglobin and the surface of the dynamic membrane. The use of high ionic strength 0.15 M NaCl involved an apparent dimerisation of myoglobin and consequently a lower permeation through the membrane due to the molecular weight increase. The permeation and retention of hemoglobin did not agree with the effect of pH observed with myoglobin (best permeation at isoelectric pH) but followed the behavior of myoglobin. This was explained by a myoglobin concentration 10 times higher than hemoglobin concentration. The yield of retention selectivity was investigated. Selectivity of the membrane at pH 8.6 and 1.5 m M was favorable t o myoglobin (increase of 40%) whereas a reversed selectivity was observed at pH 7.3, 0.15 M.