Separation of Genomic DNA from Plasmid DNA by Selective Renaturation with Immobilized Metal Affinity Capture

Separation of genomic DNA, RNA, and open

Separation of genomic DNA, RNA, and open circular plasmid DNA from supercoiled plasmid DNA by combining denaturation, selective renaturation and aqueous two-phase extraction

✍ Andreas Frerix; Petra Geilenkirchen; Markus Müller; Maria-Regina Kula; Jürgen Hu 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 377 KB 👁 2 views

## Abstract In the current study we developed a process for the capture of pDNA exploiting the ability of aqueous two‐phase systems to differentiate between different forms of DNA. In these systems scpDNA exhibits a near quantitative partitioning in the salt‐rich bottom phase. The successive recove