DNA adducts are regarded as individual internal dosimeters for the exposure to chemical carcinogens. To date, the most sensitive method for DNA adduct analysis is the radioactive 32P-postlabeling method, which allows the detection of one adduct in 10(10) unmodified nucleotides in microg amounts of D
Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis
β Scribed by Kelong Wang; Dechen Jiang; Christopher E. Sims; Nancy L. Allbritton
- Book ID
- 119289288
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 810 KB
- Volume
- 907
- Category
- Article
- ISSN
- 1873-376X
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## Abstract Phosphatidylinositol (PI) and its phosphorylated derivatives known as phosphoinositides (PIPs), are essential regulators of cell signaling and membrane trafficking, cytoskeletal dynamics, and nuclear functions. Disruption of PI metabolism is associated with disorders such as immune dysf
Capillary electrophoresis with laser-induced fluorescence detection was used to monitor biosynthetic transformations of a labeled derivative of \(\boldsymbol{N}\)-acetyllactosamine ( \(\beta \mathrm{Gal}(1 \rightarrow 4) \beta \mathrm{GlcNAc}\), LacNAc) in crude microsomal extracts. For this purpose