Separation of fluorescently derivatized deuterated isotopomers of phenylalanine using micellar electrokinetic chromatography and flow counterbalanced micellar electrokinetic chromatography

✍ Scribed by Christopher T. Culbertson; James W. Jorgenson

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 158 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:3<175::aid-mcs2>3.0.co;2-#

✦ Synopsis

Micellar electrokinetic chromatography MEKC and flow-counterbalanced MEKC separations of phenylalanine isotopomers are demonstrated. Using MEKC in an untreated, bare, fused silica capillary, tetramethylrhodamine derivatized phenylalanine can be baseline separated from derivatized phenylalanines Ž . Ž . with five aromatic ring and eight aromatic ring, ␣ and ␤ carbon deuteriums in 3.25 min. In addition, a mixture of phenylalanine isotopomers, synthesized through a partial deuteration of the amino acid's aromatic ring, was separated using Ž . flow counterbalanced MEKC FC-MEKC . In these separations, phenylalanine isotopomers which differ by only one or two neutrons were partially resolved.