Separation of etoposide phosphate and methotrexate by capillary zone electrophoresis using UV detection with a high sensitivity cell

✍ Scribed by Dr. Yahya Mrestani; Reinhard Neubert

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 308 KB
• Volume: 19
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150191638

✦ Synopsis

Abstract

Etoposide phosphate and methotrexate are important anti‐tumor chemotherapeutic agents. Our previously presented capillary zone electrophoresis (CZE) method using a high sensitivity cell (Z‐cell) for quantitative analysis in biological media (urine, plasma) showed good precision and accuracy. The present results show that the investigation using a capillary with high sensitivity cell led to an approximately 10‐fold improvement of the detection limit compared to standard capillaries. Plasma and urine samples were analyzed by using a calibration curve for drug concentrations between 0.1 and 100 μg/mL. Good detection limits and good relative standard deviations of the migration times and of the peak areas were observed in these experiments.