Separation of DNA fragments in hydroxylated poly(dimethylacrylamide) copolymers

✍ Scribed by Marcella Chiari; Marina Cretich; Roberto Consonni

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 705 KB
• Volume: 23
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/1522-2683(200202)23:4<536::aid-elps536>3.0.co;2-#

✦ Synopsis

Separation of DNA fragments in hydroxylated poly(dimethylacrylamide) copolymers

The novel polymer matrices reported here are low-viscosity sieving media for DNA capillary electrophoresis. This new family of matrices comprises copolymers of N,Ndimethylacrylamide with different monomers which increase polymer hydrophilicity. All these new copolymers self-coat on fused-silica capillaries. Resolution, peak spacing and peak width were the parameters taken into account to assess the influence of polymer structure on separation selectivity and efficiency. This work demonstrates that the performance of polydimethylacrylamide (PDMA) can be improved through copolymerization with hydrophilic monomers. The improvement is related to the efficiency parameter. The new copolymers, due to their low viscosity high sieving capacity and ability to suppress EOF, represent a better alternative to PDMA and are suitable replaceable matrices for capillary and microchip electrophoresis.