Separation of DNA by mass-transfer properties in the presence of an electric field

✍ Scribed by Young G. Park

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 297 KB
• Volume: 7
• Category: Article
• ISSN: 1369-703X
• DOI: 10.1016/s1369-703x(00)00125-x

✦ Synopsis

Experimental and theoretical works were performed for the separation of large polyelectrolyte such as DNA in the column packed with gel particles under influence of an electric field. Since DNA quickly orients through the pores in the field direction, this paper presents how intraparticle convection affects the separation of DNAs in the column. Dimensionless transient mass balance equations were derived considering diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases and these criteria were verified using two different DNAs by electrophoretic mobilities measured experimentally, showing how the separation position of DNAs varies in the column according to values of Pe f /Pe g of individual DNA. Governing equations are solved by an operator theory and the characteristic method to yield the column response.