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Separation of dipeptides by high-resolution gas chromatography on a fused silica capillary column after trimethylsilylation

✍ Scribed by Miral Dizdaroglu; Michael G. Simic

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
370 KB
Volume
108
Category
Article
ISSN
0003-2697

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✦ Synopsis

Separation of trimethylsilyl derivatives of over 50 dipeptides was achieved by highresolution gas chromatography using a fused silica capillary column coated with methyl silicone liquid phase. Excellent peak symmetry and reproducibility were obtained. Several pairs of sequence isomeric dipeptides could also be well separated. Application of this approach to peptide sequencing by means of the dipeptidyl aminopeptidase-gas chromatography/mass spectrometry method is also discussed.

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## Abstract The general gas chromatographic retention behavior of cyclic methylsiloxanes partially substituted with phenyl or 2‐cyanoethyl groups has been systematically studied, with pyrolysis‐gas chromatography being utilized to form the cyclic siloxanes from the corresponding polysiloxanes at a