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Separation of dansylated 17β-estradiol, 17α-estradiol, and estrone on a single HPLC column for simultaneous quantitation by LC–MS/MS

✍ Scribed by Szabolcs Szarka, Vien Nguyen, Laszlo Prokai…

Book ID: 120752223
Publisher: Springer
Year: 2013
Tongue: English
Weight: 280 KB
Volume: 405
Category: Article
ISSN: 1618-2650
DOI: 10.1007/s00216-013-6710-y

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## Abstract A sensitive LC/MS/MS method has been developed by derivatization of 17β‐estradiol (E2) with dansyl chloride to quantitate 17β‐E2 in female rat serum. The use of E2‐d~5~ minimized interferences from endogenous 17β‐E2 in order to achieve a limit of quantitation (LOQ) of 2.5 pg/ml using 15