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Separation of cytochrome P-450 containing vesicles from the midgut microsomal fraction of Manduca sexta

✍ Scribed by J.J. Neal; M. Reuveni


Book ID
113380384
Publisher
Elsevier Science
Year
1992
Weight
835 KB
Volume
102
Category
Article
ISSN
0306-4492

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The suitability of Ca\*' ions for the precipitation of the microsomal fraction from the hydrocarbon-grown yeast Candida tropicalis was evaluated. In the final procedure the microsomes were precipitated by the addition of 16 mM CIaClr. Crude extracts obtained from cells via spheroplast lysis were cen