Separation of collagens by capillary zone electrophoresis

✍ Scribed by Z. Deyl; V. Rohlicek; M. Adam

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 545 KB
• Volume: 480
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84306-5

✦ Synopsis

Collagen (types I, II, V, IX and XI) constituting polypeptide chains and their polymers and cyanogen bromide-cleaved peptides of collagen type I and type III were investigated by means of capillary zone electrophoresis.

Separations were effected in 2.5 mM sodium tetraborate buffer in less than 15 min. A 50 cm x 0.1 mm I.D. fused-silica capillary was used. The separations were run at 18 kV per capillary. The results of the separation were monitored at 220 nm with an on-tube detection system. Using the Offord equation, relative retention times of cyanogen bromide cleavage fragments were plotted against A4 ' -"/Z, were A4 is the molecular mass of a polypeptide and Z its valency. A linear relationship was observed. Collagen a-chains and their polymers were also satisfactorily resolved.