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Separation of cholesterol esters. A comparison between paper and thin layer chromatography

✍ Scribed by Jiří Tichý; Sven J. Dencker

Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
563 KB
Volume
33
Category
Article
ISSN
1873-3778

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✦ Synopsis

A method is presented for direct chromatography of serum on paper, treated with silicic acid, for the separation of cholesterol esters. No previous lipid extraction is necessary. A new solvent system for' the thin-layer chromatographic separation of natural cholesterol esters is also discussed. The results obtained by paper chromatography and thin-layer chromatography are compared. Quantitative results obtained by thin-layer chromatography (heating with ammonium sulphate) show lower scatter, yet paper ~chromatography (staining with Rhodamine B) gives satisfactory results and both methods can be used for semiquantitative estimations. RF values of both methods ,and their variations for the separate results are shown.

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