Separation of biotin and its analogs by high-performance liquid chromatography: Convenient labeling for ultraviolet or fluorimetric detection

✍ Scribed by Paul-Louis Desbene; Suzy Coustal; François Frappier

• Book ID: 102626643
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 282 KB
• Volume: 128
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90386-x

✦ Synopsis

d-Biotin, dethiobiotin, d-biotin sulfoxides, and d-biotin sulfone are converted to uv-absorbing or fluorescent derivatives by reaction with w4dibromoacetophenone

or 4-bromomethyl methoxy coumarin. High-performance liquid chromatographic separation conditions of these esters are described using C,s bonded phase.