Separation of basic, hydrophilic peptides by reversed-phase ion-pair chromatography: II. Analytical applications with particular reference to phosphoserine peptides

✍ Scribed by Bengt Fransson; Ulf Ragnarsson; Örjan Zetterqvist

• Book ID: 102627439
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 370 KB
• Volume: 126
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90125-7

✦ Synopsis

Phosphoserine peptides, obtained by phosphorylation of synthetic precursors with cyclic AMP-dependent protein kinase, can be efficiently separated from the corresponding nonphosphorylated peptides and from each other by ion-pair high-performance liquid chromatography. All experiments were performed under isocratic conditions on a C,s column, using phosphate buffers with pH 3.2-4.5, n-hexane sulfonic acid as counter ion, and ethanol as organic modifier.