Separation of antigen-specific lymphocytes. A new general method of releasing cells bound to nylon mesh

✍ Scribed by H. Kiefer

• Book ID: 102823574
• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 505 KB
• Volume: 5
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830050909

✦ Synopsis

Abstract

Inserting a disulfide bridge between nylon and hapten or antigen allows the release of fiber bound cells by cleaving the disulfide bond with 10^−4^ M 2‐mercaptoethanol at 4°C. Four to 12‐fold enrichments in specific precursor cells could be obtained. The method is compared with the thermal release previously used. The T and B cell content of the specific fraction was determined.

It is suggested that the low enrichment in plaque‐forming cells of the hapten‐specific fraction found after stimulation with antigen in vitro might be due to the fact that there is also enrichment for suppressor cells in the specific fraction.